Who knows sixteen?
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16 maneh is the weight of mor, k'tzi'ah, shibolet neird, and kharkom in the incense
Sixteen are the cases when a co-wife is exempt from chalitzah or yibbum, according to Levi (Yevamos 10b) - he adds one case to the fifteen mentioned in the Mishnah (ibid. 2a).
Sixteen is the width of a street.
On Pesach we spill out a bit of the Kos by the Seder 16 times. 10 Makos. 3 times by Dam V'Aish Vsimros Ashan. 3 times by Detzach Adash B'Achav. (Orach Chaim 473 - Rama)