Who knows two?
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According to one Ladino version of Echad Mi yodea (there is more than one version) "Two are Moshe and Aharon" which resolves the seeming duplication of "Two are the Tablets" and "Ten are the Commandments"
Two are:
2 is the minimum number for a plural word that is darshoned (expounded) in the Talmud.
Two are the Chief Rabbis of Israel.
And, as as a result of the settlement brokered by Rabbi Isacc Elchanan Spektor in 1835, two were the Rosh Yeshivas of Volozhin.
Two (ט"ו) equals 15. (Suggested by my 9-year-old son.)
Two are the years that the kings of the House of Saul reigned (see: I Samuel 13:1 and II Samuel 2:10).
Two are the months before a wolf is born.
(See Dewey, T. and J. Smith. 2002. "Canis lupus", Animal Diversity Web. Accessed March 17, 2010)
(Comparison with Talmud Bavli Bechorot (PDF) 8a requires investigation.)
Two are the wives of Lemech, and Hevel according to one medrash (Bereishis Rabbah 22:3).
סימני כשרות לבהמות - Signs that an animal is Kosher
מעלה גרה - chews cud
מפרסת פרסה - split hooves
The gemara in Bava Kamma 29b states:
2 things that aren't "technically" in a person's property but (in terms of halachic responsibilities) the Torah makes it as if it is in a person's property:
a pit a person dug in the public domain (Choshen Mishpat 410:6)
chametz that remains in a person's possession on Erev Passover from noon onwards (Orach Chaim 443:1)
2- are the instances where the Torah accepts the testimony of just one witness (see: Rambam, Hilchos Eidus 5:2):
a) by a Sotah- so that she does not drink the bitter waters
b) by an eglah arufah- to prevent its neck from being broken
(d'rabbanan we also accept one witness who's testifying that a woman's husband died)
2 is the number of first Orthodox female rabbis.
http://jwablog.jwa.org/node/23
http://morethodoxy.org/2010/01/28/mahara%E2%80%9Dt-to-rabba/
2 hours of daylight were lost on account of Yaakov and (those) two were regained on his account. - Bereshit Rabbah 68:10 (Bereshit 28:11, 32:32)
Two women were haughty and had unpleasant names - Devorah ("bee") and Chuldah ("mole")